Master's degree project in plant cell molecular biology
COS, Heidelberg University, Germany
Available earliest from the April 1st 2020.
Autophagy is the major catabolic process underpinning sustainability of eukaryotic cells. It is the process by which cells engulf cargo destined for degradation into double-membrane vesicles, autophagosomes, deliver them to a lytic compartment (vacuole) and upcycle the products of degradation.
Elucidating the molecular machinery of plant autophagy enables understanding of how plants cope with biotic and abiotic stresses and contributes to our knowledge of plant developmental programs.
In our group we combine a unique expertise in plant vacuole biogenesis, endomembrane trafficking and autophagy. In this project we are going to investigate key steps of plant autophagosomes maturation prior to their fusion with the vacuole.
Establishing a set of transgenic lines expressing mutant forms of proteins required for plant autophagosomes maturation
Detecting dynamics of autophagy efficacy in the established lines
Planning and making genetic constructs for further mutations to elucidate the maturation mechanism in more details
Tracking autophagosomes in epidermal root cells of Arabidopsis thaliana.
You will acquire skills in:
Advanced fluorescent microscopy
Handling typical plant model organism Arabidopsis thaliana
Advanced DNA and protein molecular biology methods