RoPod is a chamber for growing and imaging Arabidopsis roots
RoPods are printed using PETG plastic and then super-glued to standard 24x60mm microscopy glass.
The chambers are reusable and very robust.
- Plants are grown and imaged within the same chamber, thus there is no mechanical stress during sample mounting
- Plant growth within RoPod requires very small volume of the medium, thus drug treatment will require small amount of compounds
- RoPods prevents samples from drying up during imaging allowing for long-term time-lapse series.
- Plants are immobilised within the chamber, thus the same plant can be easily tracked in a RoPod in multiple imaging sessions
-RoPods allows multiple biological replicates being imaged within the same chamber (up to 20 seedlings in a 1-well RoPod)
-The watertight version of RoPod allows drug treatment in chambers positioned vertically, e.g. 72h of Dex or b-estradiol induction.
RoPod prototype 1