How to print RoPods

For printing you will need:
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access to a 3D printer extruder with a nozzle 0.4 mm
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PETG filament, white or transparent, ⌀ 1.75 mm
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24 x 60 mm microscopy coverslip #1.5, i.e. 0.16-0.19 mm thick ( VWR, 630-2108).
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superglue
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.3mf or .stl file for RoPods
Step 1. Generate the g-code
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It is highly recommended to generate from the provided .3mf project files. However, we also provide .stl files
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If you prefer to use .stl, set the following parameters:
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follow the instructions for the chamber positioning
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Print settings-> Layer height = 0.1 mm
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Print settings-> Fill density = 50%
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Print settings-> Fill pattern = 3D Honeycomb
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Print settings-> Layers and Perimeters-> Vertical shells-> Perimeters = 4
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Slice the model and insert a pause after 5 mm height
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You can simultaneously print up to 17 RoPods on a Prusa MK2or MK3. However we strongly recommend to start with just one
Step 2. Clean the coverslip glasses​
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Remove dust and fat from the glasses by wiping them clean with a paper tissue soaked in acetone​
Step 3. Start the print
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Start the print and wait until it automatically pauses on the layer programmed int the Step 1.
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The extruder will move to the back left of the print bed while waiting for the user to resume the print
Step 4. ​Place the glasses into the slots
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The chambers and lids models have 0.1mm deep slots for the coverslips
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Add a drop of super glue to two corners of a glass. This will secure the glass' position during printing
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Use forceps and patience to carefully place the glass into its slot. Make sure it does not stick upwards or sideways
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Press down on the the corners with glue and hold for a few seconds. sic! wear gloves! superglueing oneself to a 90C- hot print bed is not a good way to start your day
Step 5. Resume the print
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Resume the print and make sure the the filament does not move the glass from its position and does not crack it
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When the chamber is ready make sure there is no filament sticking sideways. If needed imperfections can be removed with a scalpel
Video
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In this video you will find a quick step-by-step demonstration how to print a RoPod