How to print RoPods

For printing you will need:

  • access to a 3D printer extruder with a nozzle 0.4 mm

  • PETG filament, white or transparent, ⌀ 1.75 mm

  •  24 x 60 mm microscopy coverslip #1.5, i.e. 0.16-0.19 mm thick ( VWR, 630-2108).

  • superglue

  • .3mf or .stl file for RoPods

Step 1. Generate the g-code

  • It is highly recommended to  generate from the provided .3mf project files. However, we also provide .stl files

  • If you prefer to use .stl, set the following parameters:

  • follow the instructions for the chamber positioning

  • Print settings-> Layer height = 0.1 mm 

  • Print settings-> Fill density = 50%

  • Print settings-> Fill pattern = 3D Honeycomb

  • Print settings-> Layers and Perimeters-> Vertical shells-> Perimeters = 4

  • Slice the model and insert a pause after 5 mm height

  • You can simultaneously print up to 17 RoPods on a Prusa MK2or MK3. However we strongly recommend to start with just one

Step 2. Clean the coverslip glasses

  • Remove dust and fat from the glasses by wiping them clean with a paper tissue soaked in acetone​

Step 3. Start the print

  • Start the print and wait until it automatically pauses on the layer programmed int the Step 1.

  • The extruder will move to the back left of the print bed while waiting for the user to resume the print

Step 4. ​Place the glasses into the slots

  • The chambers and lids models have 0.1mm deep slots for the coverslips

  • Add a drop of super glue to two corners of a glass. This will secure the glass' position during printing

  • Use forceps and patience to carefully place the glass into its slot. Make sure it does not stick upwards or sideways

  • Press down on the the corners with glue and hold for a few seconds. sic! wear gloves! superglueing oneself to a 90C- hot print bed is not a good way to start your day

Step 5. Resume the print

  • Resume the print and make sure the the filament does not move the glass from its position and does not crack it

  • When the chamber is ready make sure there is no filament sticking sideways. If needed imperfections can be removed with a scalpel

Video

  • In this video you will find a quick step-by-step demonstration how to print a RoPod

 © 2021. Alyona Minina